setup.py

# -*- coding: utf-8 -*-
from distutils.core import setup
import py2exe, os
# command to run: python setup.py py2exe
##Tree:
#Desktop/
#   Project Folder/
#       assets/
#       script.py

Mydata_files = []
for files in os.listdir('C:\\Users\\User\\Desktop\\Project Folder V5\\assets\\'): ##gets list of files from the assets folder
    f1 = 'C:\\Users\\User\\Desktop\\Project Folder V5\\assets\\' + files
    if os.path.isfile(f1): # skip directories
        f2 = 'assets', [f1]
        Mydata_files.append(f2)
Mydata_files.append('C:\\Users\\User\\Desktop\\Project Folder\\config.ini') ##append any files you want to include.
icon_location = "C:\\Users\\User\\Desktop\\Project Folder\\icon.ico" ##for the exe icon

setup_dict = dict(
    name="App Name",
    version="0.0.0.1",
    description="Short Deskription of your app",
    author="Name",
    windows = [{
            "script":"script.py",
            "icon_resources": [(0, icon_location), (1, icon_location), (42, icon_location)], ##adds the icon to your exe.
            "dest_base":"AppName", ##the output folder you want to build into
            "copyright": u"Copyright © 2018-2023 Your Name Here",
            "company_name": u"You Company Name",

            }],
    zipfile=None,
    options={
         "py2exe": {
            "optimize": 2,
            "dll_excludes": [
            "api-ms-win-core-heap-l2-1-0.dll",
            "api-ms-win-core-delayload-l1-1-1.dll",
            "api-ms-win-core-libraryloader-l1-2-0.dll",
            "api-ms-win-security-activedirectoryclient-l1-1-0.dll",
            "api-ms-win-core-delayload-l1-1-0.dll",
            "api-ms-win-core-processthreads-l1-1-0.dll",
            "api-ms-win-core-string-obsolete-l1-1-0.dll",
            "api-ms-win-core-profile-l1-1-0.dll",
            "api-ms-win-core-errorhandling-l1-1-0.dll",
            "api-ms-win-core-sysinfo-l1-1-0.dll"
            ]

            }
        },
    data_files=Mydata_files
)

setup(**setup_dict)
setup(**setup_dict)##this has to run twice to deal with the exe icon issue.