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Trimming a PNG file in Microsoft Paint generally shouldn't lead to a loss of quality, as long as you're merely cropping the image and not resaving it in a different format. PNG files are lossless, so they maintain their quality regardless of how many times you edit and save them.

However, if you resave the file in a format like JPEG, you might experience some quality loss due to compression. So, just stick to cropping in the same format, and you should be good!