SELECT route_id
FROM insert_route
WHERE
INSERT_ID IN ( 21 )
AND route_id
IN ( 48, 49, 44, 50, 55, 43, 51, 25, 24, 52, 47, 31, 53 )
AND route_id NOT
IN (

SELECT route_id
FROM insert_route
WHERE INSERT_ID =12
)
GROUP BY route_id
HAVING count( DISTINCT
INSERT_ID ) =1